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    Bio-Rad ufc501096 colloidal gold total protein stain bio rad
    Ufc501096 Colloidal Gold Total Protein Stain Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ufc501096+colloidal+gold+total+protein+stain+bio+rad/Colloidal+Gold+Total+Protein+Stain/pm30639242-237-21-27
    Average 94 stars, based on 192 article reviews
    ufc501096 colloidal gold total protein stain bio rad - by Bioz Stars, 2026-10
    94/100 stars

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    Staining:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    Magnetic Beads:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    Recombinant:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    Cloning:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    Bicinchoninic Acid Protein Assay:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    Isolation:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    Polymerase Chain Reaction:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    In Situ:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    Labeling:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    Viability Assay:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    RNA Sequencing:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    Sequencing:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    Gene Expression:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    CRISPR:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    Mass Spectrometry:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    Expressing:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019

    Mutagenesis:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. REAGENT or RESOURCE SOURCE IDENTIFIER ANTI-FLAG M2 Affinity Gel Millipore Sigma Cat# A2220 Amicon Ultra-0.5 mL Centrifugal Filters Millipore Sigma Cat# UFC501096 Colloidal Gold Total Protein Stain Bio-Rad Cat# 1706527 InstantBlue Protein Stain Expedeon Cat# ISB1L GDP Abcam Cat# ab146529 Pierce Anti-HA Agarose Thermo Fisher Scientific Cat# 26182 Pierce Anti-HA Magnetic Beads Thermo Fisher Scientific Cat# 88837 Torkinib (PP242) Selleck Chemicals Cat# S2218 GST-Akt1tail-AA409-480 GenScript Custom Production H-Ras (WT) (human), (recombinant) [IP-Kinase] Enzo Life Sciences Cat# BML-SE131-0100 Recombinant H-Ras (G12V) protein (GST Tagged) [IP-Kinase] Abcam Cat# ab90627 AP21967 (A/C Heterodimerizer) Takara Cat# 635055 KU-0063794 Selleck Chemicals Cat# S1226 BD Matrigel Matrix BD Biosciences Cat# 354234 H-Ras protein: His tagged: human wild-type (MST) Cytoskeleton, Inc Cat# RS01 Critical Commercial Assays MycoAlert Mycoplasma Detection kit Lonza Cat# LT07-318 In-Fusion HD Cloning Plus Takara Cat# 638909 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Colloidal Blue Staining Kit Thermo Fisher Scientific Cat# LC6025 DNeasy Blood and Tissue Isolation Kit QIAGEN Cat# 69506 OneStep PCR Inhibitor Removal Kit Zymo Cat# D6030 NucleoSpin Gel and PCR Clean-Up Takara Cat# 740609.250 Duolink In Situ Detection Reagents Orange Millipore Sigma Cat# DUO92007 Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat# MO-L008 TruSeq RNA Library Prep Kit v2 Illumina Cat# RS-122-2001 CellTiter-Blue Cell Viability Assay Promega Cat# G8080 Deposited Data Cell Line RNA Sequencing Data This paper Sequence Read Archive: SRP156923 HT-1376 RNA Sequencing Data Cancer Cell Line Encylopedia https://portals.broadinstitute.org/ccle/ users/sign_in Caco-2 RNA Sequencing Data Gene Expression Omnibus (GEO) repository GEO: GSE48603 Ras Proximity Proteomics Peptide Counts This paper Table S1 CRISPR Screen Unique Guide Counts This paper Table S2 Crosslinking Mass Spectrometry Data This paper Table S4 Primary Melanocyte Gene Level Expression Data This paper Table S5 Mutation and expression analysis of melanoma. .. Catalogue of Somatic Mutations in Cancer (COSMIC) v.72 https://cancer.sanger.ac.uk/cosmic Raw Images of Data This study, Mendeley Data https://doi.org/10.17632/kzxnrmh7fc.1 Experimental Models: Cell Lines Human: CHL-1 ATCC CRL-9446 Human: HEK293T Lab stock N/A Human: HT-1376 ATCC CRL-1472 (Continued on next page) e3 Molecular Cell 73, 1–15.e1–e12, February 21, 2019



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